C. elegans RNAi Services

​RNA interference (RNAi) in Caenorhabditis elegans has significantly advanced genetic research by offering a versatile and efficient method for gene silencing, thereby enhancing our understanding of gene function and regulation.​

In C. elegans, RNAi is initiated when double-stranded RNA (dsRNA) is introduced into the
organism. The dsRNA is processed into short interfering RNAs (siRNAs) by the enzyme Dicer. These siRNAs are then incorporated into the RNA-induced silencing complex (RISC), guiding the complex to complementary messenger RNA (mRNA) molecules, which are subsequently degraded, leading to gene silencing. ​

Several methods have been developed to induce RNAi in C. elegans, including feeding,
microinjection, and soaking techniques. A notable feature of RNAi in C. elegans is its systemic nature; gene silencing can spread from the initial site of double-stranded RNA (dsRNA) introduction to other tissues. Additionally, RNAi effects can be inherited by subsequent generations, allowing for the study of gene function over multiple life cycles.​