Tissue-specific feeding RNAi

While RNA interference (RNAi) is a powerful tool in C. elegans research, certain tissues, notably neurons, exhibit reduced sensitivity to RNAi. This reduced sensitivity is attributed to the absence of the double-stranded RNA (dsRNA) transporter SID-1 in neuronal tissues. To overcome this limitation, researchers have engineered C. elegans strains that express SID-1 specifically in neurons, thereby enhancing RNAi sensitivity in these cells. This targeted expression enables effective gene silencing within neuronal tissues, facilitating the study of neuronal gene functions. ​Building upon this approach, scientists have refined the technique by restricting SID-1 expression to specific tissues, enabling tissue-specific RNAi in C. elegans. This advancement allows for precise investigation of gene function within targeted tissues, minimizing off-target effects in non-target tissues.

Estimated Delivery Time - 4 Weeks and Up