Lipid Accumulation Assay

C. elegans is a valuable model organism for investigating lipid metabolism, providing insights into fat storage mechanisms pertinent to human health. To visualize and quantify lipid accumulation within this transparent nematode, Oil Red O (ORO) staining is commonly employed. This method involves fixing the worms and staining them with ORO dye, which binds specifically to neutral lipids, allowing for the assessment of fat storage through light microscopy.

Deliverable

1. Representative pictures of Oil Red O Staining for each strain/treatment
2. Quantify fat storage for each strain/treatment